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Dionex AminoPac PA10氨基酸分析色谱柱

更新时间:2019-11-12

简要描述:

Dionex AminoPac PA10氨基酸分析色谱柱
AminoPac PA10柱结合阴离子交换,梯度淋洗以及脉冲电化学检测,可以分离多种氨基酸。

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Dionex AminoPac PA10氨基酸分析色谱柱

用途和特点
AminoPac PA10柱结合阴离子交换,梯度淋洗以及脉冲电化学检测,可以分离多种氨基酸。分离氨基酸的同时,还可检测氨基糖及碳水化合物。此外,还可分离多种糖类,磷酸化氨基酸,以及含硫氨基酸的普通氧化产物,如磺基丙氨酸。其柱填料为疏水性高聚物薄壳型阴离子交换剂,可在pH0-14范围内使用。填料独特的pH稳定性允许使用的淋洗液,有助于氨基酸在金电极的阳极氧化。

 

 

Dionex AminoPac PA10氨基酸分析色谱柱

订货信息:

分析柱
AminoPac PA10 Analytical Column (2 x 250 mm)055406
AminoPac PA10 (22 x 250 mm)SP5488
AminoPac PA10 (9 x 250 mm)SP5490
AminoPac PA10 Analytical Column (4 x 250 mm)SP5678
保护柱
AminoPac PA10 Guard Column (2 x 50 mm)055407
AminoPac PA10 Guard Column (4 x 50 mm)SP5680
配件
AAA-Direct Installation Kit059539
Carbohydrate Removal Cartridge (CRC) for AAA-Direct068598

 

For High-Resolution Separations of Free Amino Acids by AAA-Direct

The AminoPac® PA10 column is packed with a hydrophobic, polymeric, pellicular, anion-exchange resin that is stable over the range of pH 0–14. The unique pH stability allows the use of eluents that are conducive to anodic oxidation of amino acids at gold electrodes. This column is recommended for use with the AAA-Direct Amino Acid Analyzer, allowing direct detection of primary and secondary amino acids by IPAD, with no need for pre- or postcolumn derivatization.

  • Derivatization not required

  • Carbohydrates and amino acids separated and detected simultaneously

  • Mid-femtomole to low-picomole detection limits

  • Compatible with all commonly used hydrolysis procedures

  • Can be configured for on-line analysis

AAA-Direct is a technique that eliminates the need for any form of derivatization, and is therefore compatible with on-line monitoring of cell cultures and fermentation broths. 

The AminoPac PA10 column is optimized for separations of free amino acids by AAA-Direct. Vitamins, amino sugars, and carbohydrates can be detected simultaneously with amino acids. Additional capabilities include separation of a wide range of sugars, phosphorylated amino acids, and common oxidation products of sulfur-containing amino acids, such as cysteic acid, methionine sulfone, and methionine sulfoxide.

 

 

AminoPac PA10 Column for Amino Acid Analysis by AAA-Direct  Specifications
Recommended operating temperature30 °C
Typical operating conditions0.25 mL/min gradient
Recommended flow rate: 0.25 mL/min
Mobile phase compatibilitypH 0–14; 100% compatible with common organic solvents.
Dionex sodium acetate ionic form eluents only.
Resin composition8.5-μm-diameter microporous (<10 ?) bead (55% cross-linked)
agglomerated with an 80-nm microbead alkyl ammonium functionalized latex (1% cross-linked).
Anion-exchange capacity60 μeq/column (2 × 250 mm analytical column)
Column constructionPEEK with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic.
DimensionsAminoPac PA10 Analytical Column:
2 × 250 mm
AminoPac PA10 Guard Column:
2 × 50 mm
Maximum operating pressure4000 psi (27.9 MPa)

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