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Dionex AminoPac PA1 氨基酸分析色谱柱

更新时间:2019-11-12

简要描述:

Dionex AminoPac PA1 氨基酸分析色谱柱
特别适用于分析酸性和酸不稳定性氨基酸,以及用阳离子交换分离不完全的氨基酸对。

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Dionex AminoPac PA1 氨基酸分析色谱柱

用途和特点
AminoPac PA1柱用于氨基酸的快速分离。特别适用于分析酸性和酸不稳定性氨基酸,以及用阳离子交换分离不完全的氨基酸对。

 

 

Dionex AminoPac PA1 氨基酸分析色谱柱

订货信息:

分析柱
AminoPac PA1 Analytical Column (4 x 250 mm)037022
保护柱
AminoPac PA1 Guard Column (4 x 50 mm)037023

 

 

For Specialized Amino Acid Applications with Postcolumn Derivatization

The AminoPac® PA1 column is a high-speed, pellicular, strong, anion-exchange column. The PA1 delivers high-efficiency separations as a result of its nonporous-core particle. Its strong anion-exchange characteristics are imparted by agglomerating surface MicroBead™ particles that are functionalized with the quaternary ammonium functional group, resulting in a higher capacity than typically offered by nonporous resins.

  • Resolves phosphorylated and other amino acids by anion-exchange chromatography

  • Separates acid-labile amino acids under basic pH conditions

  • Separates strongly acidic amino acids

  • Requires OPA or ninhydrin postcolumn derivatization

The anion-exchange selectivity of the AminoPac PA1 can be used to resolve peaks that cannot be resolved using a cation-exchange column. For example, O-phosphorylated amino acids are resolved and eluted near the end of a hydrolysate chromatogram. Arogenic acid, a precursor to tyrosine and phenylalanine, decomposes under the acidic conditions required for cation-exchange separation or PITC derivatization, but it is stable in alkaline eluents used with AminoPac PA1 column.

The PA1 is particularly well suited for acidic and acid-labile amino acids and for amino acid pairs not completely resolved by cation-exchange chromatography.

 

 

 

AminoPac PA1 Column for Specialized Amino Acid Applications with Postcolumn Derivatization  Specifications
DimensionsAminoPac PA1 Analytical Column:
4 × 250 mm
AminoPac PA1 Guard Column:
4 × 50 mm
Maximum operating pressure4000 psi (27.9 MPa)
Recommended operating temperatureAmbient
Typical operating conditions1.0 mL/min gradient
Recommended flow rate: 1.0 mL/min
Mobile phase compatibilityCompatible with up to 5% common organic solvents.
Recommended eluents: hydroxide, borate, and acetate.
Resin composition10-μm-diameter, pellicular substrate (2% cross-linked)
agglomerated with a 180 nm MicroBead alkyl quaternary ammonium functionalized latex (20% cross-linked).
Anion-exchange capacityN/A
Column constructionPEEK with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic.

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