多潘立酮与氟哌利多的分离-Zodiac C18 色谱柱

更新时间：2021-07-20 点击次数：1423次

Column : C18 100mm*4.6mm, 3µm
Flow Rate: 1.5ml/min
Wave Length : 280nm
Temperature: 25°C
Runtime : 20 minutes

Preparation of 流动相:

1.) : 流动相A
Dissolve 5.0g of Ammonium Acetate in 1000ml of HPLC Grade Water

2.) 流动相 : B
Methanol HPLC Grade

3.) Gradient Program :

Time (min)	流动相-A(%V/V)	流动相- B(%V/V)
0-10	70	30
10-15	0	100
15-15.01	70	30
15.01-20	70	30

4.) Diluent : N,N Dimethylformamide
Domperidone Reference Solution (a) : (0.10 and 0.15 mg/ml) Weight accurately about 10mg of Domperidone CRS and 15mg of Droperidol CRS into a 100ml Volumetric flask dissolve and dilute to volume with Diluent and mix Inject 10µl of Domperidone Reference Solution (a) in duplicate and calculate Resolution between Domperidone and Droperidol.

Acceptance Criteria:

Resolution between Domperidone and Dropridol should not be less than 2.0

Column Name : Zodiac C18 Column

Compound Name : Droperidol, Domperidone

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